Expression of ABF3 in the absence of selleck chem Enzastaurin stress is generally Inhibitors,Modulators,Libraries very low but it is rapidly induced in response to ABA. Overexpression of ABF3 would therefore be expected to initiate those pathways that are typically activated Inhibitors,Modulators,Libraries in response to stress via ABA mediated signal ling. This has been observed for other transcription Inhibitors,Modulators,Libraries fac tors and is generally used as a means of identifying targets of that particular transcription factor. Three independent plant lines containing the 35S ABF3 transgene were compared to the corresponding control plant lines from which the 35S ABF3 transgene was excised. Based on microarray analysis, using a cut off of P 0. 05, only a small number of genes were differentially expressed in the three 35S ABF3 transgenic plant lines. The only gene that was differentially expressed in all three plant lines was ABF3.

Drought response of 35S ABF3 plants Overexpression of ABF3 in Arabidopsis confers enhanced drought tolerance. Since ABF3 is a transcription factor, it is likely that this is achieved at the level of gene expression. Therefore, microarray ana lysis should provide insight into the mechanism of drought tolerance. Since the expression pattern of ABF3 during the drought response Inhibitors,Modulators,Libraries is achieved by constitutive overexpression from the CaMV 35S promoter, it is pos sible that unintended effects may also be generated downstream. By comparing the transcriptional profile of 35S ABF3 plants to control plants, it may be possible to identify these unintended effects. Two time points were examined in order to consider both early and late responses to drought.

For this analysis, the Control 48 and 35S ABF3 48 lines were selected as the Control 48 lines showed the smallest number of differentially expressed genes. Following 2 h of drought stress, using a cut off of P 0. 05 and a fold change of 2, 508 genes were differen tially expressed in the control plants exposed to drought stress compared to unstressed control plants and 468 genes were Inhibitors,Modulators,Libraries differentially expressed in 35S ABF3 plants exposed to drought stress compared to unstressed 35S ABF3 plants. Following 24 h of drought stress, 592 genes were differentially expressed in control plants exposed to drought stress compared to unstressed con trol plants and 797 genes were differentially expressed in 35S ABF3 plants exposed to drought stress compared to unstressed 35S ABF3 plants. In order to compare the profile of genes expressed in the two plant lines in response to drought stress, func tional selleck Z-VAD-FMK categorizations of the differentially expressed genes were obtained from The Arabidopsis Information Resource and com pared.