Without the effects of sedation, subjects who undergo colonoscopy with Penthrox® analgesia can be discharged

earlier, which may facilitate work-flow and improve cost efficacy of busy endoscopy units. Key Word(s): 1. methoxyflurane; 2. morbid obesity; 3. sleep apnoea; 4. colonoscopy; Presenting Author: CHANGHAO LIU Additional Authors: RUIRUI QIAO, YONGZHAN NIE, DAIMING FAN, KAICHUN WU Corresponding Author: KAICHUN WU Affiliations: Xijing Hospital of Digestive Diseases; Institute of Chemistry, selleck compound Chinese Academy of Sciences Objective: Gastric cancer is the second leading cause of cancer death all over the world. Accurate diagnosis of early gastric cancer could effectively decrease its mortality. MGb2 is a promising antibody for early gastric cancer detection with its great specificity and sensitivity. In this study, we are aimed to establish a gastric cancer specific, magnetic/optical dual-modality imaging probe Fe3O4-MGb2-Cy5.5 and verify its imaging capability. Methods: Fe (acac) 3, PEG (COOH)2, etc. were used to synthesize biocompatible Fe3O4 nanoparticle and Cy5.5 labeled MGb2 antibody was conjugated to the surface via EDC/NHS method. Probe’s physical features were determined by TEM, TGA and DLS. UV-Vis, fluorescence excitation, confocal microscopy

assays as well as Prussian’s blue staining, magnetic resonance imaging were used to verify the optical and magnetic Trichostatin A solubility dmso imaging capability, respectively. Nude mice bearing gastric cancer xenograft were used for biodistribution description and in vivo imaging. Results: Biocompatible Fe3O4 nanoparticle was successfully synthesized. Each MGb2 antibody was labeled with 3.3 Cy5.5 and three MGb2 were conjugated to one nanoparticle. After incubation

with the probe, gastric cancer cell SGC-7901 could be detected by both fluorescent and Prussian’s Blue assays, which distinguished from negative control GES cells. Intravital fluorescent imaging and MRI scanning of gastric cancer xenograft exhibited most obvious tumor imaging 24 hours after probe administration. Biodistribution assay implicated the probe concentrated well tuclazepam in tumor area and mainly metabolized through liver. Conclusion: We have successfully established a gastric cancer specific, magnetic/optical dual-modality imaging probe, which showed excellent tumor imaging capability. This could further facilitate early diagnosis of gastric cancer utilizing multi-modality molecular imaging. Key Word(s): 1. Molecular imaging; 2. Gastric cancer; 3. MGb2; 4. Dual-modality; Presenting Author: LING XING Additional Authors: DONG WANG, DONGZHEN JIN Corresponding Author: LING XING, DONG WANG, DONGZHEN JIN Affiliations: the General Logistics Department Objective: The holmium laser has three parameters: energy, frequency and time. Exploring the range of holmium laser in different energies, different frequencies and different times act on porcine pancreas in vitro.