The VAS score of subjects’ anal discomfort
were higher when inserting 3D-HARM catheter than HRAM and WPAM catheters as well as the VAS score of rectal discomfort. By vector analysis of the pressure morphology of the two patients with dyssynergia provided by the three systems, we determined that 3D-HARM provided greater anatomic detail for anorectal motility disorder. Conclusion: Most pressure measurements and rectal sensation except for anal sphincter pressures were consistent among the three systems. Patients tolerated better with WPAM and HRAM, while 3D-HRAM provided greater anatomic detail. Key Word(s): 1. three-dimensional; Ku-0059436 order 2. high resolution; 3. water-perfused; 4. anorectal manometry; Presenting Author: SONG XIANG Additional Authors: ZHANGLING DOCTOR, YUHONG GANG Corresponding Author: SONG XIANG Affiliations: wuhan university; Wuhan University Objective: To study the expressions and significance of Notch1 and p-Akt in colon cancer. Methods: The Osimertinib in vivo expression of Notch1 and p-Akt gene in 30 colon cancer tissues and
30 adjacent non-cancerous tissues were assayed by immunohistochemical method and western-blot method, and analyze the relationships among the expression of Notch1, p-Akt and the clinicopathological characteristics of colon cancer. Results: The positive rate of Notch1 was significantly higher in colon cancer tissues than adjacent non-cancerous colon tissues (70% vs. 30%, P < 0.05), and the expression of Notch1 was closely related to differentiation grade (P < 0.05). The positive rate of p-Akt was significantly higher in colon cancer than adjacent non-cancerous colon
tissues (83.33% vs. 16.67%, P < 0.05), and the expression of p-Akt was closely related to differentiation grade and lymph node metastasis (P < 0.05). There was positive correlation between the expression of Notch1 and p-Akt protein 上海皓元医药股份有限公司 in colon cancer (r = 0.441, p = 0.017). Conclusion: The abnormal expression of Notch1 and p-Akt may correlate to the occurrence and development of colon cancer. Key Word(s): 1. Notch1; 2. p-Akt; 3. Colon cancer; Presenting Author: XIAHONG YOU Additional Authors: YUN TAN Corresponding Author: YUN TAN Affiliations: renmin hospital of Wuhan University; renmin hospital of Wuhan University Objective: To study the effect of small interference RNA (siRNA) silencing RIP1 on the biological behavior of human colorectal carcinoma cell line LoVo and provide the basis evidence to the feasibitity of colorectal cancer gene therapy. Methods: To culture human colorectal cancer LoVo cells in vitro, LoVo cells were divided into three groups: the RIP1 siRNA group, the bland control group and the negative control group.