HE staining was used to observe the distribution of CIK and tumor cells. Further, antitumor activity of CIK cells was examined in nude mouse xenograft model. Ten nude mice were injected with 6 × 106 TE3 cells subcutaneously. Five days later, CIK cells (5 × 107) (experiment group) or BPS (control group) was injected into nude mice intravenously once a week. Results: The CIK cell population contained 97.39% CD3+ cells and 39.8% CD3+CD56+ cells. At the effector-target cell ratio of 30:1, CIK cells killed nearly 50% of TE3 cells. HE staining showed CIK cells aggregated around TE3 cells when they were co-cultured. In nude mice model,

tumor weight was reduced in CIK cells LDE225 cell line treated group compared with control group (0.21 ± 0.07 g vs. 0.53 ± 0.10 g, P < 0.05). Here, we provide evidences that CIK cells had an growth inhibition effect on esophageal squamous cells carcinoma in vitro and in vivio. Conclusion: CIK cells therapy may be a candidate choice for esophageal cancer patients. Key Word(s): 1. esophageal cancer; 2. immunotherapy; 3. CIK cells; Presenting

Author: LINLIN REN Additional Authors: JIE HONG, JINGYUAN FANG Corresponding Author: JIE HONG, JINGYUAN FANG Affiliations: Renji Hospital, Shanghai Jiao-Tong University School of Medicine Objective: The polycomb group protein EZH2, which has histone methyltrasferase (HMT) activity, has been increasingly studied recently. It was reported that EZH2 is involved in many processes www.selleckchem.com/products/Bortezomib.html such as cell cycle, apoptosis. However, whether EZH2 participates in the process of authphagy and its regulatory mechanism in CRC (colorectal cancer) remains unclear. Methods: ZEB1, EZH2 and PTEN expression were measured by Western Blot and immunohistochemistry respectively. ZEB1, EZH2 and PTEN mRNA level were measured by real-time PCR. Transfection of ZEB1 siRNA, EZH2 siRNA and other plasmids were carried out by using Lipofectamine 2000. Chromatin Immunoprecipitation (ChIP) assay was performed using the ChIP assay system. Luciferase reporter gene assay was carried out using the Dual-Glo® Luciferase Assay

System following the manufacturer’s protocol. Results: Knockdown of EZH2 induced the formation of autophagesomes in colorectal cancer cell lines HCT116 and SW620, which was evident on electron microscopy. Furthermore, Western 上海皓元医药股份有限公司 Blot and real-time PCR data showed that ZEB1 and EZH2 may regulate the expression of PTEN, which plays a vital role in autophagy. Moreover, downregulation of ZEB1 significantly reduced the expression of EZH2. A highly inverse correlation between the expression of EZH2 and ZEB1 and that of PTEN was also revealed in CRC tissues compared with normal tissue in patients Conclusion: we firstly revealed the impact of EZH2 on autophagy during CRC carcinogenesis. At the same time, we firstly identified that EZH2 expression may be regulated by ZEB1 in colorectal cancer.