MR and MV independently scanned all retrieved citations based on title and abstracts. Subsequently, the full texts of articles of relevant abstracts

were retrieved. Ten relevant studies were selected for the purpose of this investigation (Cameron et al. 2009; Cameron and Muller 2009; Condit 2001; Harel et al. 2003; Henneman GW3965 clinical trial et al. 2004, 2006; Sanderson et al. 2004; Sussner et al. 2009; Tercyak et al. 2006; Toiviainen et al. 2003). From these studies and from our personal experience, we formulated 22 items that could influence the use of a genetic test. The items were clustered in 10 domains and processed in a topic list (“Appendix 1”). The 10 domains were: (1) expected use of genetic test (results); (2) test content; (3) feelings and emotions; (4) involvement with HE; (5) principles/beliefs; (6) expected effects of HE; (7) relative risk of developing HE; (8) accessibility, safety and privacy; (9) practical considerations and (10) social influence and media. All three involvement methods comprised two parts and started with an introduction on the purpose of the study, the time schedule and confidentiality. During the first part, following the introduction, a hypothetical “case” was presented in which a genetic test for susceptibility to HE was introduced (Fig. 1). This presentation was concluded by two questions: (1) Would you use this test? (yes, no or doubt) and (2) What are your motives for using or not using this test? (open question).

Barasertib in vivo In the focus groups and interviews, answers were first Morin Hydrate noted by the participants and were subsequently discussed. During the second part, we introduced

and discussed a topic list with items extracted from the literature. Participants were asked if (yes or no) and how (open question) the different items of this topic list would influence their choice to use this test. The items that had already been discussed during the first part were not reviewed. After this discussion, participants were invited to mention supplemental items. Fig. 1 Case: a genetic test for susceptibility to hand eczema. The case was used to guide the focus groups, interviews and questionnaires Before application, the focus group protocol, interview protocol and questionnaire were all piloted. Additionally, a draft version of the electronic questionnaire was tested on comprehensibility among four workers from the Academic Medical Center in Amsterdam, the Netherlands. By convenience sampling, we recruited one worker from the catering service, one from the transport service and two student nurses. The focus groups were held between October and December 2009 and were moderated by MR. MV participated as the case presenter and www.selleckchem.com/products/mm-102.html observer. Both researchers had been trained in qualitative methods. Focus group sessions lasted for about 2 h and were audio-recorded. Five to eight student nurses participated in each group, numbers depending on availability for the scheduled time. Participants received a gift coupon with a value of €20,–.