Protein concentrations were continuously monitored by optical den

Protein concentrations were continuously monitored by optical density (280nm). DNA, gly-cosaminoglycans (GAGs) and collagen contents were assessed and a hematoxylin and eosin (H&E) staining was performed. Results: After 24h the liver had a white and clear appearance and protein levels didn’t rise anymore. DNA was significantly decreased in the decellularized tissue (1.9% only SDS, 0.5% SDS and DNase) while GAGs could be preserved.

Collagen levels decreased to 51% in SDS + DNase flushed liver and to 32.9% in SDS flushed livers respectively. H&E staining showed an intact extra cellular matrix with no nuclear residuals (Figure). Conclusion: This study shows

that porcine Vismodegib datasheet livers can be successfully decellularized with low volumes of a 1% SDS solution and DNase in a standardized process in only 24 hours, in order to obtain an organ scaffold which can be used for tissue engineering and later on for transplantation. The loss of collagen might be critical for recellularization attempts but needs to be tested in further settings. Portal trias of a decellularized liver, H&E staining Disclosures: The following people XL184 order have nothing to disclose: Nicola Buehler, Martin Schenk Aim: Efforts to identify cell sources and approaches for cell therapy of liver diseases are ongoing. The aim of the present study was to evaluate the feasibility, safety and the clinical outcomes of the first procedure of transplantation of human fetal biliary tree stem cells in patients with advanced liver cirrhosis.

Methods: The cells were immune-sorted for EpCAM (Epithelial Cell Adhesion Molecule) from human fetal biliary tree (including the gallbladder) by protocols in accordance MCE with current good manufacturing practice (cGMP). Cell products were evaluated by standard sterility tests for gram+, gram-, aerobic and anaerobic bacteria, mycetes and with endotoxins tests, and characterized immediately by Flow Cytometry (FC) for EpCAM and Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 5 (LGR5) before transplantation. Two patients with advanced cirrhosis (Child-Pugh C) have been submitted to the procedure of via hepatic artery cell transplantation and observed trough a 12 months follow-up. Patients were not candidates for liver transplantation because of their age being greater than 70 years. Informed consent was obtained from the patients. Results: In fetal tissues, most EpCAM-positive cells co-expressed LGR5 and were located in the ductal plate, in the surface epithelium and bud of peribiliary glands of larger intrahepatic and extrahepatic bile ducts, and in gallbladder epithelium.

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