Recruitment was conducted from 5 October 2007 to 31 March 2009 through 38 sites across the province of Ontario and is reviewed in detail elsewhere [14]. An attempt was made to stratify recruitment by provincial regions described by the provincial Public Health Departments such that the study sample would be proportional to the geographical distribution of the HIV-positive female population in Ontario [14,15]. Each research site received ethics approval from their local institutional research ethics board. Written informed

consent was obtained from every OSI-744 supplier participant. A 189-item survey instrument, The HIV Pregnancy Planning Questionnaire, was created using the methods of Fowler for instrument development and has been previously described in detail elsewhere (full survey instrument available upon request) [14,16]. The survey was first developed in English and translated into French using the back translation method.

Content and face validity were achieved as previously www.selleckchem.com/products/Bortezomib.html described [14]. Baseline characteristics of the study population were summarized using medians and interquartile ranges (IQRs) for continuous variables and frequencies and proportions for categorical variables. The primary outcome of interest for this analysis was unintended pregnancies. The question in the survey used to represent unintended pregnancy was ‘Was your last pregnancy planned?’ The variable was dichotomized into ‘unintended pregnancy’ if answered ‘No’ and ‘intended pregnancy’ if answered ‘Yes’. Women who had never been pregnant were excluded from the analysis. Women who had been Ribonucleotide reductase pregnant but did not answer this question or answered

‘I don’t know’ were also excluded from the analysis. Additional analyses were carried out limiting the sample to those with pregnancies before and after HIV diagnosis. Other outcomes of interest included the total number of births, the proportion of women who gave birth before and after their HIV diagnosis and the timing of births. Univariate logistic regression models were fitted to determine the unadjusted odds ratios with 95% confidence intervals (CIs) for correlates of unintended pregnancy after HIV diagnosis. Current CD4 cell count, viral load, employment status, household income, sexual relations and contraceptive use were not considered in the regression models as they corresponded to the time of administration of the survey and not the time of the last unintended pregnancy.