Site-specific conjugation of Df-Chx-Mal to thio-trastuzumab was complete within 1 h at pH 7.5, while Df-lac and Df-Bac respectively required 2 and 5 h at pH 9.

Each Df modified thio-trastuzumab was chelated with Zr-89 in yields exceeding 75%. Zr-89-Df-Ac-thio-trastuzumab and Zr-89-Df-Chx-Mal-thio-trastuzumab were stable in mouse serum and exhibited comparable PET imaging capabilities in a BT474M1 (HER2-positive) breast cancer U0126 concentration model reaching 20-25 %ID/g of tumor uptake and a tumor to blood ratio of 6.1-7.1.

Conclusions: The new reagents demonstrated good reactivity with engineered thiol groups of trastuzumab and very good chelation properties with 89Zr. The site-specifically Zr-89-labeled thio-antibodies were stable in serum and showed PET imaging properties comparable to lysine conjugates. (C) 2010 Elsevier Inc. All rights reserved.”
“The stability of 6 reference genes, 18S, beta-actin, RPS20, eEF1 alpha, G6PDH and BMS-754807 chemical structure GAPDH, was examined in tissues from Atlantic salmon (Salmo salar) and Chinook salmon embryo cells (CHSE-214). The main objective of this study was to determine the most suitable reference genes for use for the normalisation of data in quantitative real-time RT-qPCR assays conducted on infected tissues. The tissue samples selected for analysis were taken from

head kidney and pylorus and collected at different time points during a challenge experiment with infectious pancreatic necrosis virus (IPNV). The stability of some of the reference genes was also studied in infected CHSE-214 cells. The ranking of the genes examined was carried out using the geNorm program. This program determines the most stable genes from a set of genes tested in a given cDNA sample. The stability of the reference genes varied in different tissues and in the cell line at different stages of infection with IPNV. This study demonstrated that check details tissue-specific combinations of reference

genes must be used to normalise real time data for use for the quantitation of IPNV. (C) 2009 Elsevier B.V. All rights reserved.”
“Purpose: To evaluate the efficiency of baculovirus vectors in transducing FTC-133 cells and to examine the feasibility of using baculovirus vectors for the delivery of the sodium-iodide symporter (NIS) gene as a reporter through co-transduction to monitor the expression of the target gene.

Method: Two recombinant baculoviruses were constructed to express NIS and green fluorescent protein (GFP) respectively. FTC-133, 8050C, SW1116. A549 cells, were infected with Bac-GFP. The infection efficiency of Bac-GFP and the intensity of fluorescence, in either the presence or absence of sodium butyrate, were monitored by flow cytometry. The iodine uptake by FTC-133 cells infected with Bac-NIS was measured using a gamma counter. FTC-133 cells were infected with a mixture of equal amounts of Bac-NIS and Bac-GFP at different setting of multiplicity of infection (MOI).