The contribution of microRNAs in translational gene control has been established however; the role of microRNAs in disrupting the cap-dependent translation regulation complex has not been previously described. Here, we established that elevated miR-520c-3p represses global translation, cell proliferation and initiates premature senescence in HeLa and DLBCL cells. Moreover, we demonstrate that miR-520c-3p directly targets translation

initiation factor, eIF4GII mRNA and negatively regulates eIF4GII protein synthesis. miR-520c-3p overexpression diminishes cells colony formation and reduces tumor growth in a human xenograft mouse model. Consequently, downregulation of eIF4GII by siRNA decreases translation, cell proliferation and ability to form colonies, as well as induces cellular senescence. In vitro GW-572016 molecular weight and in vivo findings were further validated in patient samples; DLBCL primary cells demonstrated low miR-520c-3p levels with reciprocally up-regulated eIF4GII protein expression. Our results

provide evidence that the tumor suppressor effect of miR-520c-3p is mediated through repression of translation while inducing SBE-β-CD inhibitor senescence and that eIF4GII is a key effector of this anti-tumor activity.”
“Eating frequency has been implicated in the risk of colorectal cancer (CRC) in several epidemiological studies with contradictory and inconclusive findings. We performed a meta-analysis to evaluate their relationship. The pooled relative risk (RR) with 95 % confidence interval (CI) was calculated to estimate the effects. A total of 15 eligible studies with 141,431 subjects and 11,248 cases were retrieved after

a comprehensive search of the PubMed, Cochrane Library, and Web of Science databases up to October 2013. The overall meta-analysis revealed no strong significant association between eating frequency and risk of CRC in different eating occasion categories (1 meal/day): RR = 1.01, 95 % CI 0.94-1.09, P = 0.709; 3 vs. smaller than 3 daily meals: RR = 1.17, 95 % CI 0.93-1.46; 4 vs. smaller than 3 daily meals: RR = 1.13, 95 % CI 0.92-1.38; a parts per thousand yen5 vs. smaller than 3 daily meals: RR = 0.95, buy LBH589 95 % CI 0.61-1.47; 4 vs. a parts per thousand currency sign3 daily meals: RR = 1.18, 95 % CI 0.92-1.51; and 1-2 vs. 3 or 4 daily meals: RR = 0.82, 95 % CI 0.63-1.06). However, modest evidence of an increased risk of CRC in case-control studies (RR = 1.30; 95 % CI, 1.11-1.52) and a parts per thousand yen5 vs. a parts per thousand currency sign3 meals group (RR = 1.30; 95 % CI, 1.11-1.52) was observed. Our meta-analysis results do not support the hypothesis that eating frequency strongly reduced or increased the risk of CRC. Clinical randomized trials are required to evaluate this relationship further.