The number of down regulated genes ranged from 4. 7% in NTERA 2 R NTERA 2 to 37. 7% in NCCIT R NCCIT and 53. 3% in 2102EP R 2102EP cell line pairs. Thirty four overlapping genes were found in the two pairs NTERA 2 R NTERA 2 and NCCIT R NCCIT. The overlap ping number of differentially expressed genes was only 5 between cell line blog of sinaling pathways pairs NTERA 2 R NTERA 2 and 2102EP R 2102EP, and 6 between NCCIT R NCCIT and 2102EP R 2102EP. Only hsa miR 10b cell lines exposed to increasing concentrations of cisplatin resulted in a 2. 7 11. 3 increase in IC50 to the substance, as measured by the MTT assay 2,5 Diphenyltetrazolium Bromide. Whereas the IC50 values of the sensitive parental cell lines were 0. 45 uM, 0. 75 uM, and 1. 75 uM, respectively, the three cisplatin resistant sublines exhibited IC50 values of 5.

1 uM in NTERA 2R, 4. 9 uM Inhibitors,Modulators,Libraries in 2102EP R, and 4. 7 uM in NCCIT R. Results of the cytotoxicity experiments have been reported else where. and has miR 512 3p species were found to be up regu lated in all three cell lines. Taken together, NTERA 2 R NTERA 2 and NCCIT R NCCIT shared the vast majority of their differentially altered micro RNAs, whereas 2102EP R 2102EP showed a rather different profile. A complete list of all detect able miRNA examined is provided as additional file. Discussion Our approach Inhibitors,Modulators,Libraries enabled us to investigate almost all cur rently known human micro RNAs simultaneously. Quantitative RT PCR was chosen because of its recog nized precision and superiority over other methods for gene expression analysis.

Our experiments were run in triplicate and on average revealed a CV 30% covering variability caused by methodological, intra and inter individual sources. Since this CV is clearly below the defi nition of a two fold threshold for differential gene expression, we are convinced that the results of our ana lysis represent true differences in micro RNA expression between the Inhibitors,Modulators,Libraries cell line pairs. Inhibitors,Modulators,Libraries Of 738 micro RNA species examined, only 9. 8% appeared differentially expressed with considerable over lap in micro RNA gene expression occurring between the cell line pairs NTERA 2 R NTERA 2 and NCCIT R NCCIT, but considerably less overlap with the cell line pair 2102EP R 2102EP cells. Functionally, the most striking distinguishing feature between the cell lines NTERA 2 and NCCIT on the one hand, and 2102EP on Inhibitors,Modulators,Libraries the other hand is a difference in their capacity to differentiate.

Whereas the former two www.selleckchem.com/products/Dasatinib.html lines can differentiate upon stimuli such as exposure to retinoid acid, the latter is nullipotent and is therefore devoid of alterations induced by processes of cellular differentiation. Interestingly, there is increasing evi dence for a link between cellular differentiation and resistance to chemotherapy in germ cell tumors, both in cell line models, as well as in primary tumor material, which seems to be linked to two microRNA clusters, namely miR371 373 and miR302.