tored in NeuroExplorer for offline analysis. HC-030031 For each stimulus site, peri stimulus time histograms of most nerves were calculated using NeuroExplorer, and exported to Matlab for further analysis as in our previously published work. Active sensorimotor stimulation procedure Along with the passive sensory stimulation procedure, an effective sensorimotor stimulation procedure was performed twice for every single animal: once after an of saline and once after an injection of medicine, five minutes before the stimulation procedure began. This process consisted of producing single neuron activity whilst the dog locomoted on the motorized treadmill, similar to our previous work. The rat was placed on the low moving treadmill in an closed chamber whilst the single neuron discrimination process, as defined above, was done at each station. A video camera was put into a position Cellular differentiation which permitted a view of the rat during treadmill locomotion, once the individual neuron discriminations were complete. A mirror was placed behind the pet and the lateral view of the rear of the rat was also noted. The camera was linked to a VCR, which captured 60 frames per second. The VCR was linked to a signal generator and time/date text inserter that noted the period of the rats awake, freely moving period with millisecond resolution on each figure. At the start of the neuronal recording, the clock was reset to zero by the Plexon MNAP programs start recording TTL heart synchronizing the video with the neural information. Sensory indicators and synchronized MAPK phosphorylation high speed movie were recorded simultaneously through the entire recording session. The treadmill was switched on to run at a speed of 6. 5 m/min. Neural recordings were started, after the animal started treadmill caused locomotion and the video recording was synchronized by the Plexon system with neural information. Each recording session lasted 10 minutes. Off line video analysis of behavior The videotape of each recorded session was seen off line, one frame at a time, to spot the time at which each forepaw made connection with the treadmill. The timestamp on that frame was entered in to the NeuroExplorer data file containing the times of action potentials for every individual cell saved, once the correct frame was determined. For each recording session, the occasions of the initial 100 forepaw footfalls for each foot were identified. Data analysis A similar method was used to examine the responsiveness of neurons to both the passive or active excitement. For both, its not all cell responded to stimulation. Only cells that showed a substantial reaction were used for further analysis. Peri stimulus time histograms were made around professional, to ascertain if your cell had an important reaction