Under a microscope with transmitted light, the light absorption routine of a seminiferous tubule fits with defined levels of the wave, causing the differential appearance of tubule segments. Thus, the various stages of rodent spermatogenesis can be recognized and collected for morphometric and biochemical analysis utilizing a transillumination assisted microdissection technique. The accuracy of natural compound library the isolation of particular stages may be improved by mixing it with phase contrast microscopy of living cell squash preparations. Moreover, the collected tubule segments from mouse testicular tubules may be cultured in-vitro up to 7 days without influencing germ cell differentiation or normal progression through different developmental stages of spermatogenesis. Like, diplotene spermatocytes from late meiotic prophase endure meiotic divisions and produce haploid post meiotic spermatids all through an in-vitro culture of 2 3 days. This technology allows analysis of various drug or toxin effects on spermatogenesis. After drug incubations, the testicular cells are sent from the section and a monolayer is prepared for live Inguinal canal cell analysis or the cells are processed for biochemical assays. We used the Aurora kinase drug ZM447439 to testicular tubules in culture, to study the implications of the inhibition of Aurora kinases in the testis. We discovered that both MI and MII spermatocytes addressed with ZM447439 present similar chromosomal and spindle disorders that simulate the noted Aurora B depletion phenotype in somatic cells. These results suggest that Aurora kinases play important roles during male meiosis where they promote appropriate microtubule chromosome attachments during both MII and MI, regulate meiotic spindle gate signaling, and are necessary for normal cytokinesis. All substances and reagents were from Sigma unless otherwise indicated. DMSO, nocodazole, taxol, ZM447439, and MG132 were utilized in different studies at 70 nM, 5 uM, 100 nM, 2 20 uM, and 20 uM concentrations, respectively. ZM447439 was a gift from AstraZeneca. All data shown are put from independent studies. Flupirtine All animal studies were approved by the Turku University Committee on the Ethics of Animal Experimentation. Adult male Sprague Dawley rats were sacrificed by cervical dislocation under CO2 anesthesia. The testes were removed and detunicated. On the basis of the appearance produced by the different light absorption under a microscope, seminiferous tubules were dissected into pieces of defined stages in a dish containing PBS. Dissected 1 2 mm tubule sections were collected and transferred in a ul aliquot of medium onto a microscope slide.