When a large amount of homogeneous animal modes are required in experiments, especially in new antitumor drug tests, this method of tumor tissue injection promises the capacity to meet the demands. Acknowledgements This work was funded by grants from the national Basic Research Program of China (973 Program:2010CB529403) LY2109761 manufacturer and the Natural Science Foundation of China (NO. 30872654, 30772241), and the Natural Science Foundation of Jiangsu Province (NO. BK2007507, BK2008173). References 1. LY3023414 datasheet Rygaard J, Povlsen CO: Heterotransplantation of a human malignant tumour to “”Nude”" mice. Acta Pathol

Microbiol Scand 1969, 77:758–760.PubMedCrossRef 2. Mickey DD, Stone KR, Wunderli H, Mickey GH, Vollmer RT, Paulson DF: Heterotransplantation of a human prostatic adenocarcinoma cell line in nude mice. Cancer Res 1977, 37:4049–4058.PubMed 3. Candolfi M, Curtin JF, Nichols WS, Muhammad AG, King GD, Pluhar

GE, McNiel EA, Ohlfest JR, Freese AB, Moore PF, Lerner J, Lowenstein PR, Castro MG: Intracranial glioblastoma models in preclinical neuro-oncology: neuropathological characterization and tumor progression. J Neurooncol 2007,85(2):133–148.PubMedCrossRef BI 2536 clinical trial 4. Tabuchi K, Nishimoto A, MatsumotK O, Satoh T, Nakasone S, Fujiwara T, Ogura H: Establishment of a brain-tumor model in adult monkeys. J Neurosurg 1985,63(6):912–916.PubMedCrossRef 5. DeArmond SJ, Stowring L, Amar A, Coopersmith P, Dougherty D, Spencer D, Mikkelsen T, Rosenblum M: Development of a non-selecting, non-perturbing method to study human brain tumor cell invasion in murine brain J Neurooncol. 1994, 20:27–34. 6. Engebraaten O, Hjortland GO, Hirschbert H, Fodstad O: Growth of precultured human glioma specimens in nude rat brain. J Neurosurg 1999, 90:125–132.PubMedCrossRef 7. Yamada S, Khankaldyyan V, Bu MYO10 X, Suzuki A, Gonzalez-Gomez I, Takahashi K, McComb JG, Laug WE: A method to accurately inject tumor cells into the caudate/putamen nuclei of the mouse brain. Tokai J Exp Clin Med 2004, 29:167–173.PubMed 8. Jia Zf, Pu PY, Kang CS, Wang GX,

Zhang ZY, Qiu MZ, Huang Q: Effect of SEPT7 on the malignant phenotype of transplanted glioma in nude mice. Chin J Oncol 2008, 30:3–7. 9. Taillandier L, Antunes L, Angioi-Duprez KS: Models for neuro-oncological preclinical studies:solid orthotopic and heterotopic grafts of human gliomas into nude mice. J Neurosci Methods 2003, 125:147–157.PubMedCrossRef 10. Antunes L, Angioi-Duprez KS, Bracard SR, Klein-Monhoven NA, Le Faou AE, Duprez AM, Plénat FM: Analysis of tissue chimerism in nude mouse brain and abdominal xenograft models of human glioblastoma multiforme: what does it tell us about the models and about glioblastoma biology and therapy? J Histochem Cytochem 2000, 48:847–858.PubMed 11.