91 1.93 1.9 1.93 1.8 1.67 1.94 1.94 1.92 2.37 1.97 2.01 2.44 1.74 2.01 2.01 1.84 2.35 1.96 2.01 2.47 1.69 2.01 2.01 Trichophyton rubrum (8) a 47 50 53 69 53 53

78 69 50 75 88 75 63 63 88 75 63 50 63 63 63 38 63 50 b 1.11 1.13 1.28 1.43 1.47 1.29 1.51 1.65 1.52 1.27 1.35 1.5 1.64 1.54 1.63 1.83 1.35 1.07 1.38 1.46 1.47 1.81 1.54 1.74 Trichophyton soudanense (6) a 17 17 71 38 46 13 92 88 0 17 67 50 50 0 100 100 0 50 67 50 50 17 83 100 b 1.08 1.24 1.39 1.46 1.37 1.17 1.91 1.97   1.35 1.48 1.53 1.42   2 2.02   0.96 1.03 1.08 1.21 0.86 2 1.9 All buy LXH254 species in the library (177) a 68 64 Cell Cycle inhibitor 70 74 70 67 83 87 73 72 80 80 75 72 88 90 73 69 72 72 69 68 84 88 b 1.56 1.58 1.64 1.73 1.65 1.65 1.92 1.96 1.7 1.7 1.73 1.82 1.75 1.78 2.01 2.05 1.58 1.57 1.64 1.72 1.67 1.63 1.94 2 Non-A. SB273005 in vitro Table 4 Modulation of the database performance for independent spots regarding the MSP creation parameters Libraries LS1 mean Nb. of peaks parameter B1 1.34 449 1.58 361 1.04 0.38 25 70 B1b 1.34 449 1.58 361 1.04 0.38 25 100 B1c 1.34 449 1.58 361 1.04 0.38 50 70 B1d 1.36 473 1.60 337 1.03 0.40 50 100 B1e 1.34 449 1.58 361 1.04 0.38 75 70 B1f 1.32 445 1.56 365 1.03 0.36 75 100 B1g 1.39 473 1.63 337 1.05 0.43 100 70 B1h 1.39 473 1.63 337 Urease 1.05 0.43 100 100 B7 1.80 611 1.96 199 1.30 0.53 25 70 B7g 1.80 595 1.96 215 1.35 0.50

100 70 Considering Aspergillus fumigatus isolates separately, the results ranged from 79% (B0/B1) to 97% (B7) concordant identifications, whereas for other species, the percentage of concordant identification ranged from 56% (B0/B1) to 79% (B7) (Table 3). Finally, the identification of a clinical isolate, regardless of the species, was not improved by creating metaspectra (MSP) of the 4 spectra for the comparison of the various libraries (Table 3). The multivariate analysis findings (Table 5) indicate that concordant identification rates increased significantly with the number of both RMS per strain and raw spectra per RMS. Similarly, the LS values significantly increased (p<10-4) with the independent effect of the numbers of RMS per strain and raw spectra per RMS (data not shown).