MASK and MMS held PhD and Post-doctoral fellowships from CNPq, re

MASK and MMS held PhD and Post-doctoral fellowships from CNPq, respectively Electronic supplementary material Additional file 1: Figure S1: Circular dichroism spectrum of purified H. seropedicae His-PhbF. Figure S2: Gel filtration chromatography of purified H. seropedicae His-PhbF. Figure S3: Schematic organization of genes

probably involved in polyhydroxyalkanoate (PHA) pathway and regulation in H. seropedicae. #selleck randurls[1|1|,|CHEM1|]# Figure S4: The DNA-binding assays of purified His-PhbF from H. seropedicae to the nifB promoter region (negative control). (DOC 261 KB) References 1. Anderson AJ, Dawes EA: Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev 1990,54(4):450–472.PubMed 2. Madison LL, Huisman GW: Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol Mol Biol Rev 1999,63(1):21–53.PubMed 3. Jendrossek D: Polyhydroxyalkanoate granules are complex subcellular organelles (carbonosomes). J Bacteriol 2009,191(10):3195–3202.PubMedCrossRef 4. Keshavarz T, Roy I: Polyhydroxyalkanoates: bioplastics with a green agenda. Curr Opin Microbiol 2010,13(3):321–326.PubMedCrossRef

5. Kadouri D, Jurkevitch E, Okon Y: Involvement of the reserve material poly-beta-hydroxybutyrate in Azospirillum brasilense stress endurance and root colonization. CBL0137 in vivo Appl Environ Microbiol 2003,69(6):3244–3250.PubMedCrossRef 6. Ratcliff WC, Kadam SV, Denison RF: Poly-3-hydroxybutyrate (PHB) supports survival and reproduction in starving rhizobia. FEMS Microbiol Ecol 2008,65(3):391–399.PubMedCrossRef 7. Hervas AB, Canosa I, Santero E: Transcriptome analysis of Pseudomonas putida in response to nitrogen availability. J Bacteriol 2008,190(1):416–420.PubMedCrossRef 8. Babel W, Ackermann JU, Breuer U: Physiology, regulation, and limits of the synthesis of poly(3HB). Adv Biochem Eng Biotechnol 2001, 71:125–157.PubMed 9. Steinbuchel A, Hein S: Biochemical and molecular basis of microbial synthesis of polyhydroxyalkanoates in

microorganisms. Adv Biochem Eng Biotechnol 2001, 71:81–123.PubMed 10. Griebel R, Smith Z, Merrick JM: Metabolism of poly-beta-hydroxybutyrate. Florfenicol I. Purification, composition, and properties of native poly-beta-hydroxybutyrate granules from Bacillus megaterium . Biochemistry 1968,7(10):3676–3681.PubMedCrossRef 11. Potter M, Steinbuchel A: Poly(3-hydroxybutyrate) granule-associated proteins: impacts on poly(3-hydroxybutyrate) synthesis and degradation. Biomacromolecules 2005,6(2):552–560.PubMedCrossRef 12. Potter M, Muller H, Steinbuchel A: Influence of homologous phasins (PhaP) on PHA accumulation and regulation of their expression by the transcriptional repressor PhaR in Ralstonia eutropha H16. Microbiology 2005,151(Pt 3):825–833.PubMedCrossRef 13. Kuchta K, Chi L, Fuchs H, Potter M, Steinbuchel A: Studies on the influence of phasins on accumulation and degradation of PHB and nanostructure of PHB granules in Ralstonia eutropha H16. Biomacromolecules 2007,8(2):657–662.

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