Therefore, the surfaces of various A. fumigatus morphotypes differ form each other and, consequently, the reaction of host cells may vary towards divergent A. fumigatus growth forms [40]. Our findings suggest that infected hosts can discriminate between inactive RC and active potentially-invasive SC. The data are consistent with findings showing that SC (the mature form of A. fumigatus), but not RC-activated NF-kβ, stimulated pro-inflammatory cytokines

and the production of reactive oxygen by host macrophages PLX4032 datasheet [40]. Moreover, the presence of the hBD2 peptide in the respiratory cells was investigated. Detection of the hBD2 peptide by immunofluorescence in A549 and 16HBE cells exposed to the different forms of A. fumigatus confirmed its inducible expression in the infected cells. The presence of the negatively-stained cells in the infected culture may be due to Tozasertib mw defensin synthesis in the subpopulation of the epithelial cells or because of the release of synthesized defensins by the activated cells. The detection of the beta-defensin hBD2 peptide in the individual unstimulated control cells is in agreement with the observation made for the alpha-defensins; it has been reported

that individual untreated HL-60 cells may contain variable amounts of alpha defensin, as assessed by immunostaining [41]. Undoubtedly, inducible expression of defensin by cells exposed to A. fumigatus may represent the recruitment of additional cells that would Dichloromethane dehalogenase synthesize antimicrobial peptides LY2603618 in vivo and further upregulation of defensin synthesis in cells that originally contained defensin. Punctuated distribution of peptide in the

cytoplasm of A549 and 16HBE cells with a concentration in the perinuclear region was similar to the staining of defensin expressed by human gingival epithelial cells exposed to cell wall extract of the gram-negative periodontal bacteria, Fusobacterium nucleatum [33], suggesting that the mechanism of defensin expression may be universal for the different infectious agents. The punctuated perinuclear pattern of immunostaining may be related to the localisation of hBD2 in the endoplasmic reticulum and Golgi apparatus, which is in agreement with the previous observations of Rahman et al., showing that the hBD2 peptide was expressed in rough endoplasmic reticulum, the Golgi complex and cytoplasmic vesicles of human colon plasma cells [42]. Quantification of the cells stained with anti-hBD2 antibody revealed that SC induced a greater number of cells that synthesized hBD2, compared to RC and HF. Analysis of hBD2 levels in the supernatants of A549, 16 HBE and primary culture HNT cells confirmed this observation; significantly higher hBD2 levels were detected in all tested cell supernatants exposed to SC, compared to those exposed to RC, HF or latex beads.