94) The cell counts obtained for each ROI in the different secti

94). The cell counts obtained for each ROI in the different sections of each animal were averaged to calculate the mean number of c-Fos-positive cells within a particular brain region of that animal. These average

values/brain region of each animal were used for statistical analysis. Statistical evaluation of the results DAPT molecular weight was made with SPSS 20 (SPSS Inc., Chicago, Illinois, USA). In general, the data were analyzed by one-way or two-way analysis of variance (ANOVA), as appropriate, in some cases for repeated measurements. Two-way ANOVA was performed with the NOD agonists (VEH, MDP, FK565) and LPS (VEH, LPS) as the between subject variables in order to reveal significant main factor effects or interactions denoted as NOD × LPS interactions. The homogeneity of variances was assessed with the Levene test. In case of sphericity violations the Greenhouse–Geisser correction was applied. Post-ANOVA analysis of group differences was performed with the Tukey HSD (honestly significant difference) test, when the variances were homogeneous, and with the Games–Howell test, when the variances were unequal. In

case of a non-parametric distribution of the parameters, statistical differences among groups were determined with the Kruskal–Wallis test and post-hoc analysis of group differences was performed with the Mann–Whitney test. p values were adjusted for multiple comparisons with the Bonferroni correction. Probability values of p < 0.05 were regarded as statistically significant and p < 0.1 were regarded Enzalutamide clinical trial as a trend. All data are presented as means + SEM, n referring to the number of mice in each group. MDP, FK565 and LPS altered locomotion, exploration, food intake and SP in a compound-, combination- and time-dependent manner (Fig. 2). Repeated measures ANOVA revealed a significant interaction of NOD (VEH, MDP, FK565) × LPS (VEH, LPS) × time (days post-treatment) for the variation in locomotion

(F(5.661,116.05) = 2.457, p < 0.05). The same was true for exploratory behavior (F(5.250,110.25) = 2.470, p < 0.05). Likewise, there was a significant NOD × LPS × time interaction for the differences in food intake (F(5.025,105.52) = 5.244, p < 0.001). SP depended on time (F(1.130,39.55) = 27.838, p < 0.001), with a significant interaction pheromone with LPS (F(1.130,39.55) = 18.397, p < 0.001) and an interaction with the NOD agonists by trend (F(2.260,39.55) = 2.339, p = 0.10). Post-hoc analysis revealed significant NOD × LPS interactions on day 1 and 2 post-treatment. While MDP (1 mg/kg) and FK565 (0.001 mg/kg) alone did not induce any significant changes in locomotion, LPS (0.1 mg/kg) led to a decrease of locomotion for 2 days after injection when compared with the VEH-treated group. Combination of MDP + LPS attenuated locomotion compared to treatment with MDP or LPS alone during day 1 and 2 post-treatment (Fig. 2A). Likewise, the combination of FK565 + LPS significantly decreased locomotion when compared with FK565 or LPS alone.

For comparison purposes, these analyses were repeated for the dup

For comparison purposes, these analyses were repeated for the duplicate pairs in which neither video was presented with clinical details. Analysis of variance with terms for investigator and pair type were used to compare absolute differences. Reliability ratios for the UCEIS and overall severity, and intraobserver agreement at the descriptor level, were calculated as described previously. Bowker’s test for symmetry 11 tested for presentation order effects (ie, impact of viewing videos with clinical details before or after the blinded

version) on responses to descriptors. Two additional methods for calculating the Natural Product Library UCEIS were examined: 1. A normalized sum was used, in which descriptors were combined so as to contribute equally, as one-half “vascular pattern” plus one-third “bleeding” and one-third “erosions and ulcers”; the range of normalized UCEIS scores was then 0 to 3, with 17 possible scores. The design of this study did not permit a direct evaluation of the UCEIS in terms of sensitivity to change between videos at the individual patient level. Nevertheless, the data can be analyzed to assess the power of differentiation across patients (videos). All possible pairings of the 57 videos were formed, for a total of 1596 distinct pairings. Each video was evaluated by between 6 and 15 investigators in the main analysis

set. For each pair, mean differences in the UCEIS and overall endoscopic severity on the VAS, and 2-sample t tests for differences between videos for evaluation of overall severity on selleck screening library the VAS and the UCEIS were calculated. Proportions of significantly different scores (confirmed Dichloromethane dehalogenase by t tests) were studied globally and as a function of the difference in endoscopic severity on the VAS. To compare the UCEIS with established clinical measures for UC, Spearman

rank correlation tests were performed between the UCEIS and full Mayo score, partial Mayo score (excluding endoscopic evaluation),12 stool frequency/rectal bleeding, and patient functional assessment. Statistical analyses were performed using Statistical Analysis System (SAS, Cary, NC) software version 9.2. Twenty-nine investigators from 14 countries were screened for participation in the study. Eleven of the 29 succeeded on first qualification and 14 on their second attempt. One investigator failed both times, and 3 were withdrawn due to noncompliance with procedures, resulting in a total of 25 investigators (11 from North America, 9 from Central Europe, and 5 from Western Europe; see Acknowledgments). In total, 698 of the planned 700 evaluations were performed. Each video was assessed by 6 to 15 investigators. The response rate was 100% for assessment of overall severity on the VAS and for all descriptors of these 698 evaluations. The analyses that follow exclude 50 videos from the second evaluation of repeat pairs and the 100 evaluations used for clinical details/no clinical details evaluation, unless stated otherwise.

Certain Candida species are considered to be commensal organisms

Certain Candida species are considered to be commensal organisms within the oral cavity. Indeed, the prevalence of oral yeast in the general population is about 34%. 54 In 24 patients with acute periodontal infection and chemotherapy-induced myelosuppression, microorganisms were detected in high concentrations in subgingival pockets with a predominance of Staphylococcus epidermidis, C. albicans, S. aureus, and Pseudomonas aeruginosa, with combinations of these detected in some patients. 54 Raber-Durlacher et al.,55 addressed the pathogenesis of periodontal disease and the possibility of transmission of systemic subgingival microorganisms in patients with cancer treated with chemotherapy.

Those authors reported that oral infections are larger problems, mainly because there is a higher risk of infections spread from microorganisms of the mouth during the neutropenia Selumetinib occurring after chemotherapy. Thus, the inflamed periodontal tissues may act as a focus of infection, bringing significant morbidity and, in some cases can become life-threatening. Still, there is evidence that gingivitis and periodontitis are associated with fever and sepsis in these patients, because the ulcerated epithelium of periodontal pockets may serve as a route of entry of microorganisms into the bloodstream, and the propagation of systemic endotoxins and other inflammatory

mediators. Jewtuchowicz et al.56 identified different species of yeasts using www.selleckchem.com/products/VX-809.html conventional mycological methods and specific polymerase chain reaction (PCR) assays from samples at sites of periodontal disease isolated from immunocompromised patients, such as those with advanced HIV infection. Amongst 76 fungal organisms isolated, C. dubliniensis comprised 10.5% of total,

which corresponded to 4.4% of patients studied. C. albicans was the most frequently isolated species of yeast. However, Sardi et al.9 detected some species of Candida, using the PCR method, in higher quantities in diabetic patients when compared with non-diabetic patients with chronic periodontal disease. C. albicans were found in 57.3%, C. dubliniensis in 75.6%, C. tropicalis in 15.85% and C. glabrata in 4.87% of the periodontal pockets of diabetic patients. For non-diabetic patients, 19.17% and 13.69% of the periodontal sites presented C. albicans and C. dubliniensis, respectively. Calpain C. tropicalis and C. glabrata were not found in the periodontal pocket of non-diabetic patients. Urzúa et al. 57 analysed the composition of the yeast microbiota present in the mucosal and subgingival sites of healthy individuals and patients with aggressive and chronic periodontitis, using phenotypic and genotypic methods. Despite the varied profiles of the species present in the mucosa of the three groups analysed, only C. albicans and C. dubliniensis were capable of colonizing the periodontal pockets in patients with chronic periodontitis, whilst only C.

Here we report the permanent draft genome sequences of two Rhodop

Here we report the permanent draft genome sequences of two Rhodopirellula europaea strains. Strain SH398 (= IFAM 3246 = JCM 17614 = DSM 24039) was isolated by Heinz Schlesner from the Kiel Fjord, Germany (54.3297 N 10.1493 E) ( Schlesner et al., 2004), while strain 6C (= JCM 17608 = DSM 24037) originates from Porto Cesareo, Italy (40.2598 N 17.8905 E) ( Winkelmann and Harder, 2009). Other representatives of this species were also found in the North Sea, in the English Channel and at the Greek coast. The genomic DNA of both strains was extracted using the FastDNA SpinKit

for Soil (MP Biomedicals, Germany), randomly sheared into fragments (“shot gun sequencing”) and transferred into 96 well plates with 24 wells were assigned to each strain. Sequencing was performed with the Roche 454 Titanium pyrosequencing technology. The assembly was done with Newbler v. 2.3. Gene prediction was carried out by using a combination Epigenetics Compound Library of the Metagene (Noguchi et al., 2006) and GSI-IX concentration Glimmer3 (Delcher et al., 2007) software packages. Ribosomal RNA genes were detected by using the RNAmmer 1.2 software (Lagesen et al., 2007) and transfer RNAs by tRNAscan-SE (Lowe and Eddy, 1997). Batch cluster analysis was performed by using the GenDB (version 2.2) system (Meyer et al., 2003). Annotation and data mining were done with the tool JCoast, version 1.7 (Richter et al., 2008) seeking for each coding

region observations from similarity searches against several sequence databases (NCBI-nr, Swiss-Prot, Kegg-Genes, genomesDB) (Richter et al., 2008) and to the protein family database InterPro (Mulder et al., 2005). Predicted protein coding sequences were automatically annotated by the software tool MicHanThi (Quast, 2006). Briefly, the MicHanThi software interferes gene functions based on similarity searches against the NCBI-nr (including Swiss-Prot) and InterPro databases using

fuzzy logic. Particular interesting genes, like sulfatases, were manually evaluated. Both GNE-0877 genome sizes are in the range of previously reported Rhodopirellula baltica strains, with over 7 Mb and 6000 predicted open reading frames each. Pairwise analysis by reciprocal best match BLAST revealed 4700 shared genes between the two strains, with 4168 (6C) and 4376 (SH398) genes, respectively, being shared with the type strain R. baltica SH1T. This high number of shared genes reflects the close relation between the two species as predicted by 16S rDNA and ANI analysis. Compared with each other species introduced in this article series, 997 and 1039 genes, respectively, appeared to be strain specific. The number of open reading frames encoding for sulfatases, the outstanding feature of this genus, was found to be very similar in the genomes of R. europaea and R. baltica strains ( Table 1) ( Wegner et al., 2013).

“This article has been removed: please see Elsevier Policy

“This article has been removed: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). This article has been removed at the request of the author. This abstract was inadvertently published in the journal when the authors

had requested that it should not. “
“Marijuana smoke is a complex mixture composed of thousands of chemical compounds, selleck screening library many of which are qualitatively similar to those found in tobacco smoke (Moir et al., 2008). Like tobacco smoke, marijuana smoke has been associated with numerous adverse pulmonary effects in humans including airway inflammation, chronic bronchitis, edema, mucus hypersecretion, and the impairment of large airway function and lung efficiency (Lee and Hancox, 2011 and Tashkin, 2005). Moreover, Aldington et al. showed that the impairment of large airway function and lung efficiency is 2.5–5 times greater in marijuana

smokers than tobacco smokers (Aldington et al., 2007). Like tobacco smoke, previous studies have also shown marijuana smoke to be genotoxic both in vitro and in vivo (see Entinostat research buy Maertens et al., 2009 for a review). In addition, it is suspected that marijuana smoke may be carcinogenic. Indeed, some agencies such as the California Environmental Protection Agency have placed marijuana smoke on their list of chemicals known to cause cancer (Tomar et al., 2009). However, since there is a paucity of marijuana-only smoking populations to complete definitive studies, epidemiological studies conducted to date

are limited in scope, and often confounded by concurrent check details tobacco smoking (Aldington et al., 2008, Hashibe et al., 2006, Sasco et al., 2002, Sidney et al., 1997 and Voirin et al., 2006). Therefore, a clear and widely accepted empirical link between marijuana smoking and cancer does not exist. Information on the pharmacokinetics of marijuana smoke, and the mechanisms by which it may cause adverse effects, is also limited. Several mechanisms have been proposed including genotoxicity (Ammenheuser et al., 1998, Busch et al., 1979, Chiesara et al., 1983, Leuchtenberger et al., 1973, Sherman et al., 1995, Stenchever et al., 1974, Vassiliades et al., 1986 and Wehner et al., 1980), alterations in endocrine function (Lee et al., 2006 and Lee et al., 2005), alterations in cell signaling pathways (Hart et al., 2004), and immune suppression (Baldwin et al., 1997, Massi et al., 2006 and Rieder et al., 2010). However, many of these findings are based on the testing of individual cannabinoids (e.g., Δ9-tetrahydrocannabinol, cannabinol, cannabidiol) found in marijuana smoke, as opposed to the whole smoke or smoke condensate. Genome-wide expression profiling may provide information to permit a better understanding of the toxicological pathways perturbed by exposure to marijuana smoke. Currently, there are no published studies that have used a whole genome toxicogenomics approach to evaluate responses to marijuana smoke. However, Sarafian et al.

34 This speculation is supported by the findings35,

36 an

34 This speculation is supported by the findings35,

36 and 37 that reduced sensitivity of FITs for proximal colon lesions is related to hemoglobin breakdown during transit with loss of detectable epitopes. Undoubtedly, the transferability of quantitative results between different FITs can be improved through use of a standardized reporting unit system; however, findings of the present study reveal that current systems are not adequate for this purpose. In particular, antibodies provided by manufacturers of FITs are likely to differ considerably. To address this problem, the World Endoscopy Organization buy MS-275 has proposed that an independent calibration process of analytical performance is needed, in which the system under investigation is compared with an internationally accepted hemoglobin standard (eg, artificial stool material).38 and 39 Findings of the present report support this proposal. Strengths of the present study include the large sample size, long follow-up time, execution on a nationwide scale, and registry of cancer incidence and mortality, such that both short-term and long-term indicators could be evaluated. In addition to highlighting the need to improve the capacity of FITs to detect proximal CRC, findings selleck of the present study support the findings

of others40 that hemoglobin concentrations fall at higher ambient temperatures; the latter indicates the need to improve the stability of hemoglobin molecules present in fecal samples before conducting measurements. However, certain limitations of the present study should be noted. First, this study was not a randomized trial; the higher adherence rate of subjects receiving HM-Jack for diagnostic examination may have attenuated the differences oxyclozanide in the advanced adenoma detection rate and cancer detection rate between this group and those receiving OC-Sensor. In addition, their shorter follow-up time, which was related to the later marketing and selling of HM-Jack in Taiwan, may have led to an underestimation of the difference in test sensitivity between the 2 FITs. Although

regression analysis was employed in an attempt to address the baseline difference between the 2 groups, the absolute differences in test performance were small and residual confounding from measured or unmeasured factors cannot be excluded. Second, given the quantitative nature of this study, the possibility that some laboratories have adjusted the cutoff concentrations for both tests according to local screening capacities cannot be excluded. However, results in the conventional ranges of 50–100 ng hemoglobin/mL buffer for OC-Sensor and 8–12 ng hemoglobin/mL buffer for HM-Jack accounted for only 3% of both measures in the present study, and almost all interval cancers were below the defined cutoff concentrations and unlikely to alter the findings.

, 2009 and dos Santos et al , 2011a)

After the establish

, 2009 and dos Santos et al., 2011a).

After the establishment of these electrostatic interactions, the protein undergoes a quaternary rearrangement that allows hydrophobic portions of membrane phospholipids to be inserted in the protein hydrophobic channels, therefore culminating with membrane destabilization (dos Santos et al., 2011a). The first consequence of this destabilization is the loss of ionic permeability regulation, leading to a reduction of the resting membrane potential, inactivation of sodium channels and blockade of both directly and indirectly evoked contractions (Gallacci and Cavalcante, 2010). In addition, the disruption of the muscle fiber membranes induced by Lys49-PLA2s also promotes an increase of cytosolic Protein Tyrosine Kinase inhibitor calcium concentration, initiating a complex series of degenerative mechanisms that culminates with the muscle cell damage (Gutierrez and Ownby, 2003, Lomonte and Rangel, 2012 and Montecucco et al., 2008). In this article, we fully characterize functionally and structurally the Lys49-PLA2 MjTX-II from B. moojeni. Despite the fact that this class of proteins has been extensively studied, several issues regarding the function–structure relationships are still need to be clarified, as highlighted by a recent review in this field ( Lomonte and Rangel, 2012). This requirement is probably due to the high evolutionary pressure process by which snake

venom molecules are submitted, since proteins with few natural amino acid mutations 4��8C may present different oligomeric configurations, variable Cabozantinib cost toxic potency or even different functions when compared to their ancestral toxins ( Doley and Kini, 2009, dos Santos et al.,

2011b, Kini, 1997 and Kini, 2003). An interesting example is the MjTX-I, other myotoxic Lys49-PLA2 from B. moojeni that presents unusual oligomeric characteristics and displays lower myotoxic activity when compared to all other bothropic Lys49-PLA2s that have already been structurally and functionally characterized ( Andriao-Escarso et al., 2000 and Salvador et al., 2013). As demonstrated in this work, MjTX-II also presents some particularities if compared to other Lys49-PLA2s which seem to influence the mode of ligand binding along the toxin hydrophobic channel, a feature that may directly affect the design of structure-based ligands for Lys49-PLA2s. This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Financiadora de Estudos e Projetos (FINEP), Coordenação de Aperfeiçoamento de Nível Superior (CAPES) – Projeto NanoBiotec, Rede de Biodiversidade e Biotecnologia da Amazônia Legal (BIONORTE/CNPq/MCT), Instituto Nacional para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica (INCT-INPeTAm/CNPq/MCT) e Instituto Nacional para Pesquisa em Toxinas (INCT-Tox), Secretary of Development of Rondonia State (SEPLAN/PRONEX/CNPq).

Antioxidant encapsulation can be used to protect the nutritional<

Antioxidant encapsulation can be used to protect the nutritional

Copanlisib solubility dmso and sensory quality of food and/or to protect the body against chronic diseases related to aging [20•]. Fish protein hydrolysates possess antioxidant activity and the ability to scavenge hydroxyl radicals, superoxide anion radicals, hydrogen peroxide, and chelate metal ions [32]. Small peptides show higher antioxidant capacity than native proteins and may be absorbed in the intestine without further digestion. The results obtained so far suggest that the hydrolytic treatment of this industrial by-product, with selected enzymes and microbial systems, can allow its exploitation for the production of functional additives and supplements rich in antioxidant peptides, to be used in new food formulas for human consumption [18]. Mosquera et al. [23] encapsulated a collagen peptidic fraction obtained from sea bream scales subjected to enzymatic hydrolysis in nanoliposomes selleck chemical made of partially purified phosphatidylcholine obtained from industrial soy by-product. Authors as Ahn et al. (2012) [33], and Ahn

et al. (2014) [34] produced bioactive peptides from pectoral fin protein from salmon processing byproduct by enzymatic hydrolysis, and the produced hydrolysate exhibited antioxidant activity. Centenaro et al. [32] report that meat and fish provide valuable sources of protein for many populations around the world; furthermore, meat and fish proteins offer huge potential as novel sources of bioactive peptides displaying antioxidant effects. Different authors 22, 35, 36 and 37 affirm that fish proteins have properties that are advantageous in the preparation of films, such as the ability to form networks, plasticity and elasticity. Edible covers with nanoclays can extend the shelf life and improve the quality of fruits Selleckchem Abiraterone by providing barriers to mass transfer, improving integrity or handling and/or the functional loads such as antimicrobial agents

and antioxidants. El-Halal et al. (2014) [36] stated that proteins have been used extensively because of their relative abundance, nutritional qualities and film-forming ability with a good structural integrity and mechanical properties. It was interesting to investigate the effects of protein isolate and glycerol concentration and pH on the properties of protein films obtained from Whitemouth croaker (Micropogonias furnieri) residues [35]. It is also important to consider that the formation of the films involves a complex series of chemical reactions; these are influenced by experimental conditions such as protein concentration, heating temperature and the addition of a plasticizer [30].

“The notions attached with hydrological drought generally

“The notions attached with hydrological drought generally refer to shortfalls in river flows, water levels in lakes,

ponds, wetlands, ground water reservoirs, etc. By and large river flows have been used in the analysis of hydrologic droughts and therefore the term streamflow drought has also been used. One index that has become popular in recent years for identifying meteorological droughts is the standardized precipitation index (SPI), which is a seasonally (monthly, weekly, etc.) standardized-and-normalized value of the precipitation time series (McKee et al., 1993). Sharma and Panu (2010) have suggested the standardized hydrological index (SHI) as a measure for defining and modeling the hydrological droughts, which is conceptually Osimertinib mw analogous to SPI except that SHI represents a standardized value (mean, μ = 0 and standard deviation, σ = 1 of SHI sequence) which is not normalized. The distinguishing feature JAK inhibitor between a standardized-and-normalized (also called standard normal) and standardized variable is that the former is obtained by subtracting mean from the original variable, xi and division

by the standard deviation of the variable ei = (xi − μ)/σ; ei is the standardized variable and transforming it into normal distribution (ei → zi becomes a normalized variable) while in the latter case the

transformation into the normal distribution is not conducted. For example, Branched chain aminotransferase when a standardized sequence, ei is derived from a Gamma distributed variable xi; it can be transformed into a standard normal distribution, zi using Wilson–Hilferty transformation ( Viessman and Lewis, 2003). In the case of SPI, the above transformation is conducted prior to analyzing the drought parameters whereas in the case of SHI, the above transformation is not conducted. This paper describes the analysis for drought parameters using SHI as a platform. In the case of annual flow series, which is generally regarded as a case of weak stationarity, the computations for creating SHI sequences is trivial as there is only one mean and one standard deviation. In the case of monthly and weekly flow series, the creation of SHI sequences is somewhat involved because it requires stationarising the seasonal (monthly or weekly) flow series. The process of stationarising means standardization of the flow series using month by month μ’s and σ’s, that catapults into a weak stationary series with constant μ equal to zero and σ equal to one. The SHI sequence so obtained inherits the non-normal character of the seasonal flow series as no attempt is exercised to normalize it. The non-normalization offers an advantage in that the flow values are not distorted.

Ces lignes datent de 15 ans Aujourd’hui, on peut répondre que le

Ces lignes datent de 15 ans. Aujourd’hui, on peut répondre que les méthodes invasives sont de moins en moins agressives, tandis que l’ARM comme le PET-scan n’ont qu’une spécificité relative avec un certain nombre de faux-positifs et de faux-négatifs. L’apparition toute récente, il y a quelques mois de la méthode de la compression pour l’IRM avec un temps d’acquisition des images très court, de l’ordre de 15 minutes au lieu de 45 minutes avec une meilleure qualité, rend compte de la nécessité de suivre

de très près les techniques d’avenir. C’est ce que faisait Jean en assistant tous les ans à Chicago à la réunion de l’ARNA (Société de radiologie check details nord-américaine) et en rapportant ensuite devant l’Académie

de médecine les dernières nouveautés qui peuvent être des bouleversements. Mais il faut des moyens techniques pour « faire connaître le savoir » BIBF 1120 cost et le Collège français de pathologie vasculaire est fondé le 21 avril 1966 avec comme Président, le Doyen Fontaine et comme Secrétaire général, Claude Olivier. Jean en fait partie rapidement, entre au Conseil d’administration en 1968, est le Président du congrès en 1976, le Secrétaire général de 1977 à 1990 et le Président de 1990 à 2002, mais ce Collège était « SDF ». En 1998, le siège du Collège français de

pathologie MRIP vasculaire est établi 18, rue de l’Université dans le 7e arrondissement de Paris, dans des locaux que Claude Olivier avait repérés lors d’une de ses promenades dans le quartier et qui devait faire l’objet d’une prochaine vente aux enchères. Jean s’est rendu à cette vente à la chandelle et l’avait acquis, mais sa qualification de local commercial a été contestée : il aurait été occupé « bourgeoisement » quelque vingt ans plus tôt. Heureusement, grâce à votre serviteur et à la chance, nous avons pu le conserver. C’est ainsi qu’est née cette « Maison de l’angiologie » que beaucoup de sociétés nous envient. Enfin, en 1999, le siège du congrès dans des lieux historiques mais mal commodes pour une réunion qui devenait d’année en année plus importante a été transféré à la Maison de la Chimie. Il convient de rappeler dans ce bref historique le rôle essentiel de notre secrétaire, Françoise Staub, qui a accompagné le Collège pendant ces pérégrinations, ainsi que celui du cabinet Fournier qui assure tout ce côté financier que nous serions incapables de maîtriser. Cette date de 1999 est la dernière que j’ai retrouvée dans la liste des livres et des monographies qu’il adressait après leur publication à la bibliothèque de l’Académie de Médecine.