0% and a HSP coverage of 97.8%. The five most frequent keywords http://www.selleckchem.com/products/17-DMAG,Hydrochloride-Salt.html within the labels of environmental samples which yielded hits were ‘skin’ (10.3%), ‘human’ (4.9%), ‘biota, cutan, lesion, psoriat’ (4.0%) and ‘fossa’ (4.0%) (84 hits in total). Environmental samples which yielded hits of a higher score than the highest scoring species were not found. Figure 1 shows the phylogenetic neighborhood of R. anatipestifer in a 16S rRNA based tree. The sequences of the three identical 16S rRNA gene copies in the genome differ by one nucleotide from the previously published 16S rRNA sequence (“type”:”entrez-nucleotide”,”attrs”:”text”:”U60101″,”term_id”:”134093045″,”term_text”:”U60101″U60101). Figure 1 Phylogenetic tree highlighting the position of R. anatipestifer relative to a selection of the other type strains within the family Flavobacteriaceae.
The tree was inferred from 1,391 aligned characters [14,15] of the 16S rRNA gene sequence under the … The cells of R. anatipestifer are generally rod-shaped (0.3-0.5 �� 1.0-2.5 ��m) with round ends (Figure 2) . R. anatipestifer is a Gram-negative, non spore-forming bacterium (Table 1). The organism is described as non-motile. Gliding motility is not observed. Only three genes associated with motility have been found in the genome (see below). The organism is a capnophilic chemoorganotroph which prefers microaerobic conditions for growth. The optimum temperature for growth is 37��C, most strains can grow at 45��C but not at 4��C. Catalase and oxidase are present, thiamine is required for growth . R.
anatipestifer is not able to reduce nitrate and does not produce hydrogen sulfide. The organism tolerates 10% bile in serum but no growth occurs on agar containing 40% bile in serum . Many biochemical reactions are negative or strain-dependent: Hinz et al. have stated that ��R. anatipestifer is not easy to identify because it is characterized more by the absence than by the presence of specific Drug_discovery biochemical properties�� . The organism has proteolytic activity but its capacity to utilize carbohydrates is strain-dependent and has been discussed controversially. It has been described that carbohydrates are used oxidatively and that R. anatipestifer is able to produce acid from glucose and maltose, less often from fructose, dextrin, mannose, trehalose, inositol, arabinose and rhamnose . The production of indole is strain-dependent; the type strain does not produce indole . Esculin is not hydrolyzed by most R. anatipestifer strains, a trait useful for distinguishing these strains from R. columbina strains .