Cloning HpARI represents the first step towards the full characte

Cloning HpARI represents the first step towards the full characterization of HAPPY and the elucidation of the molecular mechanisms underlying

apomixis in H. perforatum.”
“Listeria monocytogenes is a pathogenic bacterium that moves within infected cells and spreads directly between cells by harnessing the cell’s dendritic actin VX-765 molecular weight machinery. This motility is dependent on expression of a single bacterial surface protein, ActA, a constitutively active Arp2,3 activator, and has been widely studied as a biochemical and biophysical model system for actin-based motility. Dendritic actin network dynamics are important for cell processes including eukaryotic cell motility, cytokinesis, and endocytosis. Here we experimentally altered the degree of ActA polarity on a population of bacteria and made use of an ActA-RFP fusion to determine the relationship between ActA distribution and speed of bacterial motion. We found a positive linear relationship for both ActA intensity and polarity with speed. We explored the underlying mechanisms of this dependence with two distinctly different quantitative models: a detailed

agent-based model in which each actin filament and branched network is explicitly simulated, and a three-state continuum model that describes a simplified relationship between bacterial speed and barbed-end actin populations. In silico bacterial motility required a cooperative restraining mechanism to reconstitute our observed speed-polarity Ferrostatin-1 cost relationship, suggesting that kinetic friction between actin filaments and the bacterial surface, a restraining force previously neglected in motility models, is important in determining the effect of ActA polarity on bacterial motility. The continuum model was less restrictive, requiring only a filament number-dependent restraining mechanism to reproduce our experimental observations. However, seemingly rational assumptions Selleck SBI-0206965 in the continuum model, e.

g. an average propulsive force per filament, were invalidated by further analysis with the agent-based model. We found that the average contribution to motility from side-interacting filaments was actually a function of the ActA distribution. This ActA-dependence would be difficult to intuit but emerges naturally from the nanoscale interactions in the agent-based representation.”
“The objective of this study was to investigate complications, urinary incontinence symptoms, and overall satisfaction in patients undergoing the tension-free vaginal tape-SECUR (TVT-S) for stress urinary incontinence (SUI).

We reviewed consecutive patients treated with TVT-S between April, 2006 and August, 2007, in a urogynecology practice. Outcomes assessed included complications, voiding function, change in SUI symptoms on the Medical Epidemiological and Social Aspects of Aging (MESA) questionnaire, and overall satisfaction.

One hundred forty-one women (age, 54.1 +/- 12 years; BMI, 31.2 +/- 6.


To assess


To assess the knowledge

of randomized clinical trials and willingness and barriers to participation among rural, remote and regional cancer patients of North Queensland.


A survey was conducted in medical oncology outpatient clinics at the Townsville and Mt Isa hospitals on patients, following their informed consent, using questionnaires. Rurality was defined according to the rural remote and metropolitan area classification.


Of the 180 patients approached, 178 participated. The median distance to the regional trial center for rural participants was 180 km (range 80-1300 km). 45.4% lived in rural or remote areas and the rest lived in Townsville, a regional metropolitan center. Their overall knowledge was low, with a median CH5424802 in vitro knowledge score of 3 (inter-quartile ranges n = 2.5). For randomized controlled trials there were no significant relationships between willingness

to participate and rurality or education level (P = 0.981). Cost of travel (41.1% rural or remote; 23.5% regional; P < 0.001) and the need for family or friends to accompany them (38.9% rural or remote; 24.1% regional, P = 0.021) were more important for rural/remote than regional patients as factors affecting participation.


Rural and remote patients are as interested in participating in randomized clinical trials as regional patients. Their knowledge of trials is poor and education earlier in the consultations is needed. Since cost of travel and the need for family members to accompany them are important for rural

patients trial budgets should include the cost of travel to encourage participation.”
“Background: During the recent decade, a number of studies have begun to address Quality of Life (QoL) in children and adolescents with mental health problems Small molecule library mw in general population and clinical samples. Only about half of the studies utilized both self and parent proxy report of child QoL. Generally children with mental health problems have reported lower QoL compared to healthy children. The question whether QoL assessment by both self and parent proxy report can identify psychiatric health services needs not detected by an established instrument for assessing mental health problems, i.e. the Child Behavior Checklist (CBCL), has never been examined and was the purpose of the present study.

Methods: No study exists that compares child QoL as rated by both child and parent, in a sample of referred child psychiatric outpatients with a representative sample of students attending public school in the same catchment area while controlling for mental health problems in the child. In the current study patients and students, aged 815.5 years, were matched with respect to age, gender and levels of the CBCL Total Problems scores.

RESULTS An unstructured model was proposed for model parameters t

RESULTS An unstructured model was proposed for model parameters that are independent of initial operating conditions, but have a highly predictive click here capability that can be used in process optimization and control. The predictions of the proposed models were compared with experimental data and against the results of other models presented in the literature. CONCLUSION A technique of gradually expanding the

search domain combined with a genetic algorithm was proposed to successfully speed up the optimization computation of parameter estimation. Compared with an earlier model presented in the literature, the proposed model shows more versatility with fewer numbers of parameters and fixed values of parameters, and provides a satisfactory prediction capability. The proposed model was used to investigate the process optimization of batch culture. Numerical results may provide various process alternatives for planning the productivity of bioethanol. (c) 2012 Society of Chemical Industry”
“Objective: The aim of the study was to evaluate factors influencing quality of life (QOL) in Moroccan

postmenopausal BAY 1895344 mw women with osteoporotic vertebral fracture assessed by the Arabic version of ECOS 16 questionnaire.

Methods: 357 postmenopausal women were included in this study. The participants underwent bone mineral density (BMD) measurements by DXA of the lumbar this website spine and the total hip as well as X-ray examination of the thoraco-lumbar spine to identify subclinical vertebral fractures. Patients were asked to complete a questionnaire on clinical and sociodemographic parameters, and osteoporosis risk factors. The Arabic version of the ECOS16 (Assessment of health related quality of life in osteoporosis questionnaire) was used to assess quality of life.

Results: The mean age was 58 +/- 7.8 years, and the mean BMI was 28.3 +/- 4.8 kg/m(2). One hundred and eight women (30.1%) were osteoporotic

and 46.7% had vertebral fractures. Most were categorized as Grade1 (75%). Three independent factors were associated with a poor quality of life: low educational level (p = 0,01), vertebral fracture ( p = 0,03), and history of peripheral fracture (p = 0,006). Worse QOL was observed in the group with vertebral fracture in all domains except “”pain”": Physical functioning (p = 0,002); Fear of illness (p = 0,001); and Psychosocial functioning (p = 0,007). The number of fractures was a determinant of a low QOL, as indicated by an increased score in physical functioning (p = 0,01), fear of illness (p = 0,007), and total score (p = 0,01) after adjusting on age and educational level. Patients with higher Genant score had low QOL in these two domains too (p = 0,002; p = 0,001 respectively), and in the total score (p = 0,01) after adjusting on age and educational level.

We present the clinicopathological and immunohistochemical featur

We present the clinicopathological and immunohistochemical features of 7 cases of intraoral MC in attempt to better understanding this entity.

Study design. Seven intraoral MCs were retrieved from the files of the Department of Oral Pathology, School of Stomatology, Wuhan University, during a 10-year period. Immunohistochemical markers, including cytokeratin

(CK) AE1/AE3, S100, vimentin, smooth muscle actin (SMA), p63, and maspin, were used to further characterize these lesions. A review of contemporary relevant literature is also provided.

Results. The patients were 5 women and 2 men, and the age range was 37 to 75 years (mean 57.9 years). selleckchem Three cases arose in the hard palate, 2 in the retromolar region, and 1 each in the tongue and the floor of the mouth. Histologically, the tumors exhibited a solid architecture with cells arranged in nodules, nests, trabeculae, or cords. The epithelioid cell type is the most frequently encountered, followed by clear cells. The tumor-associated matrix is more prevalent hyalinized than myxoid. Immunohistochemically, CK AE1/AE3, S100, vimentin, p63, and maspin were expressed in all cases. Positivity for

SMA was seen in 3 tumors. Five patients with follow-up information CA-4948 cost showed no evidence of disease after a mean follow-up of 69.6 months (range 22-108 months).

Conclusions. Combining the current series with 21 additional cases in the literature indicated that myoepithelial carcinoma of intraoral minor salivary glands is generally a low-grade malignant tumor with little propensity for regional or distant metastasis and low recurrence. Awareness of the clinicopathological and immunohistochemical features of intraoral MC is necessary for accurate diagnosis. Wide local excision with tumor-free margins is the treatment of choice. (Oral Surg Oral

Med Oral Pathol Oral Radiol Endod 2010;110:85-93)”
“Equiatomic MnAu clusters with average sizes of 4 and 10 nm are produced by inert-gas condensation. As-produced clusters are used to form both dense cluster films and films with clusters embedded in a W matrix with a cluster volume fraction of 25%. Both structure and magnetism are size-dependent. Structural analysis of the 10 nm clusters indicate a distorted tetragonal body-centered Alisertib mouse cubic structure with lattice parameters a = 0.315 and c = 0.329 nm. The 4 nm clusters have a partially ordered tetragonal L1(0) structure with lattice parameters a = 0.410 nm and c = 0.395 nm. Magnetic properties of the clusters show evidence at low temperatures of mixed ferromagnetic and antiferromagnetic interactions and ordering as well as paramagnetic spins. Saturation moments are as large as 0.54 mu(B) per average Mn atom. The results are compared with earlier theoretical calculations on bulk MnAu. (C) 2011 American Institute of Physics. [doi:10.1063/1.3559502]“
“Objective. The objective of this study was to evaluate cone-beam CT (CBCT) diagnoses of sialoliths in the major salivary glands.

Study design.

Methods A total of 24 CAPD patients (13 males, 11 females; mean

Methods. A total of 24 CAPD patients (13 males, 11 females; mean age 42.2 +/- 14.8 years) were included. Routine blood samples were examined. Left and right ventricular functions were assessed, and myocardial performance index (MPI) was calculated by SDE and TDI at baseline and after 12-month ROS therapy. Left and right atrial volumes were measured and indexed to body surface area. Results. When compared with baseline, after 12 months of ROS treatment, it was shown that AZD1152 solubility dmso early (E) and late (A) diastolic

velocities of atrioventricular valves, E/A ratio, mitral E-wave deceleration time (DT), isovolumetric relaxation time (IVRT), and MPI were similar (p > 0.05). Also, no significant changes were detected in LV dimensions, LV mass index, LVEF,

LA volume index, or RA volume index measured by SDE before and after ROS therapy (p > 0.05). Left and right ventricular function parameters measured by TDI including Sm, Em, Am, Em/Am ratio, E/Em ratio, and MPI were similar. Conclusion. It was found that there was no negative effect of long-term ROS therapy on cardiac functions measured by SDE and TDI in CAPD patients.”
“A set of proteins and noncoding RNAs, referred to as the male specific lethal (MSL) complex, is present on the male X chromosome in Drosophila and has been postulated to be responsible for dosage compensation of this chromosome e the up-regulation of its expression to be equal to that of two X chromosomes in females. This hypothesis is evaluated in view of lesser known aspects of dosage compensation such as the fact that metafemales with three X chromosomes also have equal expression to normal females, which would require a down-regulation of each gene copy. Moreover, when this complex is ectopically expressed in females or specifically targeted to a reporter in males, there is no increase in expression of the genes or targets with which it is associated. These observations are not consistent with the hypothesis that the MSL complex conditions dosage compensation. A synthesis is described

that C646 Epigenetics inhibitor can account for these observations.”
“To bind and fertilize the egg, the spermatozoon should undergo few biochemical and motility changes in the female reproductive tract collectively called capacitation. The capacitated spermatozoon binds to the egg zona pellucida, and then undergoes the acrosome reaction (AR), which allows its penetration into the egg. The mechanisms regulating sperm capacitation and the AR are not completely understood. In the present review, we summarize some data regarding the role and regulation of the epidermal growth factor receptor (EGFR) in these processes. In the capacitation process, the EGFR is partially activated by protein kinase A (PKA), resulting in phospholipase D (PLD) activation and actin polymerization. Protein kinase C alpha (PKC alpha), which is already activated at the beginning of the capacitation, also participates in PLD activation.

Furthermore, the prolonged drug release was independent of the pH

Furthermore, the prolonged drug release was independent of the pH of the dissolution media. In conclusion, SPDLAs are a novel type of drug carrier polymers Selumetinib mw applicable in oral controlled drug delivery systems.”
“Genotyping of 20 strains of Hepatitis B virus (HBV) from the Idu Mishmi primitive tribe of northeast

India identified multiple genotypes and the presence of a unique cluster grouping with strains from Vietnam and Laos identified as novel recombinants/genotype I. Sequence analysis (similarity and bootscan plots) of three complete HBV genomes from the tribe provided evidence of recombination. Phylogenetic analyses supported recombination between genotypes A, G and C. The Pre-S gene between nt 2943 and 397 was clearly of genotype A origin, whereas nt 397-1397 represented genotype G and nt 1397-2943 represented genotype C. Percentage divergence from genotypes B, D, E, F, G and H varied from 9.2 SYN-117 clinical trial +/- 0.45% to 13.8 +/- 0.53%, whereas genotype A and C differed by 7.9 +/- 0.42% and 7.4 +/- 0.39% respectively. The identification of similar recombinant viruses in three countries, especially in a primitive tribe with no contact with the outside world suggests that these viruses do not represent recent recombination events, but circulation of closely related viruses highly divergent from known HBV genotypes and should be classified as members of

genotype ‘I’.”
“The operation of a MEMS-based micro heat engine at resonant and off-resonant conditions is presented. Both model and experiment are used to investigate resonant and off-resonant operation of the engine. In this work, we look at the pressure-volume (PV) diagrams of an engine operated at resonance and off-resonance. Model predictions of the PV diagram are in favorable agreement with measured data. The results show that resonant operation is beneficial. At resonance, the pressure and volume in the engine cavity are decoupled and more mechanical selleck kinase inhibitor work is observed. The PV diagram describes an elliptical shape. However, for

an off-resonant operation the pressure and volume become more coupled and less mechanical work is observed. The PV diagram is described by a sigmoidal shape. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3372755]“
“The aim of this study was to evaluate the ecto-adenosine deaminase (E-ADA) activity in erythrocytes of lambs experimentally infected with Haemonchus contortus, correlating it with the degrees of anemia of the experimental animals. A total of 14 healthy lambs, with negative fecal exam for parasites, were to carry out the present study. They were divided into two groups, composed by seven animals: Group A represented the healthy animals (uninfected), while in Group B the animals were infected with 15,000 larvae of H. contortus.

9 +/- 26 5 minutes) When total

9 +/- 26.5 minutes). When total Metabolism inhibitor procedure and patient fluoroscopy times were divided into EPS time, time to first application, to successful application, and time to perform TS puncture or to retrogradely cross the aortic valve, only the last measurement differed significantly for both groups (P < 0.01). Ablation times were comparable in both groups. No major complications occurred.

Conclusions: Our data show that TS and TA approaches are equal in success rate and total procedure,

patient fluoroscopy, and ablation time when using the MNS for left-sided AP ablation. However, crossing the aortic valve with the MNS is faster than completing a TS puncture. (PACE 2010; 1298-1303).”
“Horticultural production has primarily focused on increasing productivity through intensification of fertilizers and water, resulting in high environmental costs. Recent research indicates that high nitrogen applications can have significantly negative effects on produce quality as well as secondary plant metabolite and vitamin

content within fruits and vegetables. Consumer awareness of both sustainable production practices and nutritional content within fruits and vegetables has risen dramatically in recent years, indicating that there is a likely market for produce grown with minimal nitrogen and water inputs. This TGF-beta inhibitor review investigates the effects on produce quality of nitrogen and water applications in fruit and vegetable crops, with specific emphasis on the content of flavonoids, carotenoids, glucosinolates and ascorbic acid. The link between the nutritional quality of horticultural crops and the environmental and social sustainability of reducing nitrogen and water is considered and discussed. Crown Copyright (c) 2010 Published by Elsevier Ltd. All rights reserved.”
“Recognizing that certain biological functions can be associated with specific DNA sequences has led various fields of biology to adopt the notion of the genetic part. This concept provides a finer level of granularity than the traditional notion of the gene. However, a

method of formally relating how a set of parts relates to a function has not yet emerged. Synthetic biology both demands such a formalism and provides an ideal setting for testing hypotheses about relationships between DNA sequences Birinapant and phenotypes beyond the gene-centric methods used in genetics. Attribute grammars are used in computer science to translate the text of a program source code into the computational operations it represents. By associating attributes with parts, modifying the value of these attributes using rules that describe the structure of DNA sequences, and using a multi-pass compilation process, it is possible to translate DNA sequences into molecular interaction network models. These capabilities are illustrated by simple example grammars expressing how gene expression rates are dependent upon single or multiple parts.

4 +/- 4 93 versus 16 4 +/- 1 74 days) Oestradiol concentrations

4 +/- 4.93 versus 16.4 +/- 1.74 days). Oestradiol concentrations of breast cancer patients on the letrozole protocol remained much lower than those of patients on the antagonist protocol. (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.”
“The incidence of adenocarcinoma of the esophagogastric junction is increasing, but laparoscopic proximal gastrectomy is not widely accepted due to the absence of a standardized technique of reconstruction. This report selleck screening library describes a novel technique

of esophagogastric tube reconstruction in laparoscopic proximal gastrectomy for Siewert type II tumors.

Laparoscopic proximal gastrectomy, sometimes with transhiatal distal esophagectomy, was performed. After a perigastric, suprapancreatic, and lower thoracic paraesophageal lymphadenectomy, a gastric tube of 35-mm width was prepared. An esophagogastric tube anastomosis with pseudo-fornix was made with a no-knife linear stapler to prevent postoperative reflux esophagitis.

Fifteen patients with Siewert type II tumors OSI-906 cell line underwent this operation. They included six patients with early-stage cancer, six at high risk for transhiatal total gastrectomy due to several comorbidities, and three who needed palliative tumor resection. The mean operation time was 315 min. One postoperative anastomotic leak was treated conservatively, and three anastomotic stenoses were resolved with

endoscopic balloon dilatation. Postoperative 1-year follow-up endoscopy revealed four cases of reflux esophagitis that were well controlled by medication.

This new technique of reconstruction Ralimetinib MAPK inhibitor was feasible. With the advantage of a gastric tube, a tension-free anastomosis was possible even for bulky tumors that needed lower esophagectomy. Although long-term follow-up and a larger number of patients are required to evaluate long-term functional outcomes and oncological adequacy, our procedure has the potential of becoming a treatment of

choice for early-stage Siewert type II tumors and/or for some selected high-risk patients who need tumor resection.”
“The French law regulating assisted reproductive technologies forbids donor spermatozoa to be available in case of failed testicular sperm extraction (TESE) performed on the day of oocyte retrieval. This article reports the first French live birth after intracytoplasmic sperm injection of donated spermatozoa into frozen-thawed oocytes cryopreserved following failure of TESE. By reinforcing the relevance of TESE performed on the day of oocyte retrieval, oocyte cryopreservation in couples having beforehand consented to go to sperm donation will avoid cycle cancellation and potentially lead to successful live birth. Therefore, it could modify the French policy of management of patients suffering from non-obstructive azoospermia. (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

8 +/- A 175 2 vs 493 8 +/- A 245 8 pg/ml (P = 0 037), respectivel

8 +/- A 175.2 vs 493.8 +/- A 245.8 pg/ml (P = 0.037), respectively. The severity of striae gravidarum during pregnancy did not correlate with serum relaxin levels.

Lower serum relaxin levels could contribute to the occurrence of striae gravidarum during pregnancy ABT-737 cost through decreased elasticity of the connective tissue.”
“In this article, novel 2,2′-bipyridyl derivatives were synthesized and the excellent bipyridyl ligands were chosen as catalyst

to apply in the copolymerization of carbon monoxide (CO) and styrene (ST) to prepare polyketone. 4,4′-Dicarboxyl-2,2′-bipyridine(4,4′-dcpy) was synthesized by use of synthesized 4,4′-dimethyl-2,2′-bipyridine(4,4′-dmpy). The products (4,4′-dmpy and 4,4′-dcpy) were characterized by melting point, NMR, IR, GC-MS, and elemental analysis. The effects of different ligands and various reaction conditions incuding the usage of CHIR 99021 ligand, p-toluenesulfonic acid, solvent, p-benzoquinone, the CO pressure, and reaction temperature on catalytic activity of the copolymerization were investigated. The catalytic activity of 4,4′-dmpy, 4,4′-dcpy and 2,2′-bipyridine were compared. The results showed that the addition of the electron-donating

substituent can enhance catalytic activity, with the sequence as follows: 4,4′- dimethyl-2,2′-bipyridine > 2,2′-bipyridine > 4, 4′-carboxy 2,2′- bipyridine. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 123: 3292-3297, 2012″
“We applied anisotropic stress to LaMnO3+delta nanoparticles synthesized in one-dimensional pores of mesoporous silica and investigated the effects of the anisotropic strain on the nanoparticles, which consisted of ferromagnetic and antiferromagnetic particles. The nanoparticles in the one-dimensional pores were crushed without stress in the direction parallel to the silica walls that separated the pores

when the mesoporous silica containing the nanoparticles is pressurized. X-ray diffraction patterns of the nanoparticles indicated that the lattice strain increased with increasing applied pressure. The lattice constants were found to reduce anisotropically, and the values saturated above a critical strain. Both the ferromagnetic and antiferromagnetic transition see more temperatures for the LaMnO3+delta nanoparticles initially increased with increasing pressure and then remained constant at around the critical strain. These results indicate that anisotropic stress causes distortion of the shape of nanoparticles. This induces an increase in the lattice strain and the anisotropic compression of the crystal structure, which, in turn, results in an increase in the transition temperatures. (C) 2011 American Institute of Physics. [doi:10.1063/1.3624741]“
“Superparamagnetic iron oxide nanoparticles (SPIOs)-embedded chitosan microspheres were developed for magnetic resonance (MR)-traceable embolotherapy.

Using these adapted protocols, the ability of the bottle assay an

Using these adapted protocols, the ability of the bottle assay and the WHO assay to discriminate between deltamethrin-resistant Anopheles albimanus populations was compared. The diagnostic dose of deltamethrin that would identify resistance in currently susceptible populations of An. darlingi and Ae. aegypti was defined. The robustness of the bottle assay during a surveillance exercise in the Amazon was assessed.

Results: The bottle assay (using Bcl-2 inhibitor technical or formulated material) and the WHO assay were equally able to differentiate deltamethrin-resistant and susceptible An. albimanus populations. A diagnostic dose of 10 mu g a.i./bottle was identified as the most sensitive

discriminating dose for characterizing resistance in An. selleck chemicals darlingi and Ae. aegypti. Treated bottles, prepared using locally sourced solvents and insecticide formulations, can be stored for > 14 days and used three times. Bottles can be stored and transported under local conditions and field-assays can be completed in a single evening.

Conclusion: The flexible and portable nature of the bottle assay and the ready availability of its components make it a potentially robust and useful tool for monitoring insecticide resistance and efficacy in remote areas that require minimal cost tools.”
“Objective: The purpose of this study was to determine

the efficacy of the AIGIS(RX)(TM) antibacterial envelope (TyRx Pharma, Inc., Monmouth Junction, NJ, USA) designed to reduce device-related infections by incorporating minocycline and rifampin in a controlled release polymer.

Methods: An infection was established in a rabbit model by creating bilateral subcutaneous implant pockets, into which a pacing device with or without AIGIS(RX)(TM) was placed.

The incisions were closed, and a defined dose of bacteria was infused into each implant pocket. After 7 days, devices were explanted and sampled for viable bacteria by swabbing and sonication.

Results: Initial studies evaluated the ability of the AIGIS(RX) pouch to reduce Staphylococcus epidermidis (S. epi) infection in this model using clinical and quantitative microbial endpoints. Results demonstrate S. epi infection in all control samples, while no pathogens were recovered from samples with the AIGIS(RX)(TM) pouch. Systemic antibiotic LY294002 datasheet levels were undetectable. Additional studies tested the efficacy of the AIGIS(RX)(TM) pouch with additional bacterial strains, Staphylococcus capitis, Escherichia coli, and Acinetobacter Baumannii. In each case, the device and implant pocket with the AIGIS(RX)(TM) pouch was free of any signs of infection. An assessment of biofilm produced by Acinetobacter demonstrated the elimination of biofilm formation on the implanted device.

Conclusion: These results demonstrate that in this animal model, the AIGIS(RX)(TM) device reduces the risk for infection of viable pathogens within implant pockets.